Complete medium for lentivirus production

Recipe

Reagent	Quantity (for ~1 L)	Final concentration
DMEM (high glucose, with L-glutamine and phenol red, no sodium pyruvate) (Mediatech)	900 mL	90%
Fetal bovine serum (FBS), heat-inactivated (Omega Scientific)	100 mL	10%
G418 (50 mg/mL) (Sigma-Aldrich)	10 mL	500 μg/mL
L-Glutamine (200 mM) (Mediatech)	10 mL	2 mM
HEPES (1 M) (Mediatech)	10 mL	10 mM
MEM nonessential amino acids (10 mM) (Mediatech)	10 mL	0.1 mM
Sodium pyruvate (100 mM) (Mediatech)	10 mL	1 mM
Streptomycin (10,000 μg/mL)/penicillin (10,000 IU/mL) (Mediatech)	10 mL	1%
Omit the G418 antibiotic when culturing cells for virus production.