Recipe

D-loop reaction buffer (5X)

ATP (10 mM)

Bovine serum albumin (500 μg/mL)

Creatine kinase (100 μg/mL)

Creatine phosphate (100 mM)

DTT (Dithiothreitol) (5 mM)

KCl (250 mM)

MgCl2 (20 mM)

Tris-Cl (125 mM, pH 7.5)

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  1. doi:10.1101/pdb.rec11589 Cold Spring Harb Protoc 2009: pdb.rec11589-
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