Recipe

MEM salts (10X)

Reagent Quantity (for 500 mL) Final concentration
MOPS (pH 7.4) 104.65 g 1 M
EGTA 3.804 g 20 mM
MgSO4 0.602 g 10 mM
H2O to 500 mL
This solution can be stored for several months at room temperature, protected from light.

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  1. doi:10.1101/pdb.rec11608 Cold Spring Harb Protoc 2009: pdb.rec11608-
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