Recipe

MABT(L)

Reagent Quantity (for 1000 mL) Final concentration
Maleic acid 11.6 g 0.1 M
NaCl 8.77 g 150 mM
Tris base 20-30 g 0.2 M
Tween 20 1 mL 0.1%
H2O to 1000 mL
Adjust pH to 7.5 with Tris base. Filtered MABT(L) can be stored for a few weeks to several months.

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  1. doi:10.1101/pdb.rec11612 Cold Spring Harb Protoc 2009: pdb.rec11612-
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