Recipe

Blocking solution for lamprey embryos

Reagent Quantity (for 100 mL) Final concentration
Blocking reagent (10%, in 1X MAB; Roche 11096176001) 20 mL 2%
Sheep serum, heat inactivated 20 mL 20%
MABT(L) to 100 mL
This solution can be stored frozen for several months at -20°C.

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  1. doi:10.1101/pdb.rec11613 Cold Spring Harb Protoc 2009: pdb.rec11613-
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