Recipe

DAB solution

Reagent Quantity (for 33 mL) Final concentration
PBS(P) (1X) 33 mL 1X
Tween 20 16.5 μL 0.05%
DAB (3,3′-Diaminobenzidine) (Sigma-Aldrich D5905) 10 mg 0.3 mg/mL
Add one 10-mg DAB tablet to a 50-mL tube containing 33 mL of PBS(P) and 16.5 μL of Tween 20. Rock gently in the dark for ~30 min. Filter through a 0.22-μm filter to remove particulate matter. Store aliquots at -70°C or in a nondefrosting -20°C freezer. Use aliquots immediately after thawing.

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  1. doi:10.1101/pdb.rec11618 Cold Spring Harb Protoc 2009: pdb.rec11618-
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