Recipe

Glycerol solutions (50% and 70%)

Glycerol (ultrapure; Invitrogen)

PBS(P) (1X)

Prepare 50% and 70% glycerol solutions by mixing the appropriate volumes of ultrapure glycerol with 1X PBS(P). Use pH paper to ensure a pH of ~7.4. Acidic glycerol will cause rapid fading of DAB reaction products.

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  1. doi:10.1101/pdb.rec11620 Cold Spring Harb Protoc 2009: pdb.rec11620-
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