Recipe

Glycine buffer

Reagent Quantity (for 50 mL) Final concentration
Glycine (pH 2.0) 0.375 g 125 mM
Triton X-100 0.05 mL 0.1%
Dissolve the glycine in 40 mL of H2O and adjust the pH to 2.0 with concentrated HCl. Add the Triton X-100 and adjust the volume to 50 mL with H2O.

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  1. doi:10.1101/pdb.rec11625 Cold Spring Harb Protoc 2009: pdb.rec11625-
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