Recipe

Whitten’s/HEPES/PVA

109.5 mM NaCl

1.2 mM KH2PO4

5.5 mM glucose

4.8 mM L(+) lactic acid (hemicalcium salt)

0.001% phenol red

15 mM HEPES

4.7 mM KCl

1.2 mM MgSO4

0.23 mM pyruvic acid (sodium salt)

10 μg/mL gentamycin

7 mM sodium bicarbonate (NaHCO3)

0.01% polyvinylalcohol (PVA)

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  1. doi:10.1101/pdb.rec11629 Cold Spring Harb Protoc 2009: pdb.rec11629-
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