Recipe

CZBT, Ca2+- and Mg2+-free (CMF-CZBT)

85.35 mM NaCl

4.83 mM KCl

1.18 mM KH2PO4

25.12 mM sodium bicarbonate (NaHCO3)

10 μg/mL gentamicin

10 μg/mL phenol red

0.27 mM sodium pyruvate

7 mM taurine

0.11 mM EDTA

31.3 mM sodium lactate

0.1% polyvinylalcohol (PVA)

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  1. doi:10.1101/pdb.rec11631 Cold Spring Harb Protoc 2009: pdb.rec11631-
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