Protocol

Extraction of DNA from the Plant Kalanchoë daigremontiana

  1. Neelima Sinha1
  1. Department of Plant Biology, University of California, Davis, CA 95616, USA
  1. ↵1Corresponding author (nrsinha{at}ucdavis.edu).

INTRODUCTION

This protocol describes how to isolate genomic DNA from leaves and stems of Kalanchoë daigremontiana. The procedure can be applied to adult leaves, but it is best to use younger leaves because they have fewer secondary metabolites and polysaccharides, which can interfere with the DNA extraction. The resulting DNA can be used for polymerase chain reactions (PCRs), Southern blots, or other applications.

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  1. doi:10.1101/pdb.prot5304 Cold Spring Harb Protoc 2009: pdb.prot5304-
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