Protocol

Strain Maintenance of Nasonia vitripennis (Parasitoid Wasp)

  1. David W. Loehlin
  1. Department of Biology, University of Rochester, Rochester, NY 14627, USA
  1. 1Corresponding author (werr{at}mail.rochester.edu).

INTRODUCTION

Nasonia is a complex of four closely related species of wasps with several features that make it an excellent system for a variety of genetic studies. These include a short generation time, ease of rearing, interfertile species, visible and molecular markers, and a sequenced genome. Furthermore, its parasitoid lifestyle allows investigations of questions relating to parasitoid/host dynamics, host preference, and specialist versus generalist biology. It also can serve as a behavior model for studies of courtship, male aggression and territoriality, female dispersal, and sex ratio control. This protocol describes standard rearing of Nasonia vitripennis strains on Sarcophaga bullata hosts.

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