Shoot-inducing medium (pH 5.8)

Recipe

Reagent	Amount to add (for 1 L)	Final concentration
Murashige and Skoog (MS) basal salt mixture with vitamins (Phytotechnology Laboratories)	4.3 g	0.43%
Sucrose	30 g	3%
Benzylaminopurine (BAP)	1 mg	0.1%
Indole-3-acetic acid (IAA)	0.1 mg	0.01%
Agar	7.5 g	0.75%

Adjust pH of medium to 5.8 using 1 M NaOH.
Dissolve BAP and IAA in a few drops of 1 M NaOH before adding H₂O to make a working solution of 1 mg/mL.
Autoclave and cool to 50°C.
Add filter-sterilized (0.2-μm) antibiotics: 300 mg/L carbenicillin (to kill Agrobacterium LBA4404) 40 mg/L kanamycin (to select for positive transformants)
Pour ~20 mL of medium per dish into Petri dishes and store at 4°C. Medium is good for 1 mo.