Buffer C
| Reagent | Amount to add (for 200 mL) | Final concentration |
|---|---|---|
| 5 M NaCl | 4 mL | 2% |
| 1 M Tris (pH 9.7) | 20 mL | 10% |
| 1 M MgCl2 | 10 mL | 5% |
| H2O | 166 mL | 83% |
| Mix reagents by stirring at room temperature. Store solution at room temperature until needed. Prepare fresh before use. | ||
