Recipe

BM blocking solution (1X)

Reagent Amount to add (for 200 mL) Final concentration
Blocking buffer for in situ hybridization (BBISH) (10X) 20 mL 1X
Maleic acid buffer 180 mL 90%
Melt a frozen aliquot of 10X BBISH at room temperature and mix with maleic acid buffer by swirling. Store block solution at room temperature until needed. Prepare fresh before use.
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  1. doi:10.1101/pdb.rec11995 Cold Spring Harb Protoc 2009: pdb.rec11995-
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