Post-Flask Management of Yam (Dioscorea) Plantlets
- 1 African Agricultural Technology Foundation, Nairobi 00100, Kenya
- 2 AVRDC-The World Vegetable Center, Regional Center for Africa, Duluti, Arusha, Tanzania
- 3 International Institute of Tropical Agriculture (IITA), Ibadan, Oyo State, Nigeria
- 4 Department of Ecology and Evolution, State University of New York, Stony Brook, NY 11794, USA
- ↵5Corresponding author (geeta{at}life.bio.sunysb.edu).
INTRODUCTION
This protocol describes how to harden or acclimatize tissue culture-derived yam (Dioscorea) plantlets before transplanting them in the soil. Briefly, the plantlets are first put in pots with a vermiculite and peat moss mixture, and the pots are placed in a humidity chamber, where they are sprayed and watered daily. After 2 wk, the plants are transplanted to pots or plastic bags with ordinary or sterile soil and watered daily. After another 2 wk, they are transplanted into the field.
