Recipe

Elution buffer for GST

Reagent Amount to add
1 M HEPES (pH 7.0) 2.25 mL
5 M NaCl 0.9 mL
1 M DTT (dithiothreitol) 0.15 mL
Reduced glutathione (Sigma) 0.14 g
H2O to 30 mL
Adjust pH to 7.8. Prepare immediately before use.
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  1. doi:10.1101/pdb.rec11342 Cold Spring Harb Protoc 2009: pdb.rec11342-
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