Glutathione agarose resin
MATERIALS
Equipment
Centrifuge (clinical)
Rocker
METHOD
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1. Hydrate the dry resin in PBT (GST) by rocking at 4ºC for at least 3 h.
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2. Centrifuge the hydrated resin in a clinical centrifuge at 1000 rpm for 15 sec to collect the resin.
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3. Rinse the resin three times in ice-cold PBT (GST).
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4. For the final resuspension, add an equal volume of PBT (GST) (ice-cold) to make a 50% slurry of the resin.
Swelled resin can be kept at 4ºC almost indefinitely.
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5. For long-term storage, add sodium azide to 0.02% (w/v).
