Recipe

Zymolyase in P solution

P solution

Zymolyase

  • 1. Combine 10 mg of dry zymolyase with 1 mL of P solution and vortex.

  • 2. Centrifuge the mixture in a microcentrifuge at 13,000 rpm.

  • 3. Allow the precipitate to settle and use the supernatant.

  • 4. Store this solution at -20°C. It can be used more than once.

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  1. doi:10.1101/pdb.rec12000 Cold Spring Harb Protoc 2009: pdb.rec12000-

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