Triton lysis buffer (complete)

25 mM HEPES

100 mM NaCl

1 mM EDTA

10% (v/v) glycerol

1% (v/v) Triton X-100

This buffer can be made ahead of time and stored at room temperature.

Just prior to use, add the following to make “complete” Triton lysis buffer:

• 1 mM PMSF (phenylmethylsulfonyl fluoride)

• 10 mM DTT (dithiothreitol)

• 1 mM sodium orthovanadate

• 10 ng/mL leupeptin

• 1 ng/mL aprotinin

  “Complete” Triton lysis buffer can be stored at −20°C for 4 wk.