Recipe

Blocking solution for Euprymna embryos

Bovine serum albumin (BSA) (0.5%)

Marine phosphate-buffered saline (mPBS) (1X)

Normal goat serum (1%)

Triton X-100 (1%)

Best results are obtained by preparing the BSA solution freshly from powdered reagent rather than from frozen concentrated stock solutions.

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  1. doi:10.1101/pdb.rec12007 Cold Spring Harb Protoc 2009: pdb.rec12007-
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