Recipe

PTw for ISH

PBS(P) (1X)

Treat the PBS(P) with 0.1% DEPC (diethyl pyrocarbonate), autoclave, and store at room temperature. DEPC alkylates histidine sidechains of RNase and other esterases.

Tween 20 (0.1%)

Filter-sterilize the final solution through a 0.2-μm filter. Store at room temperature.

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  1. doi:10.1101/pdb.rec12011 Cold Spring Harb Protoc 2009: pdb.rec12011-
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