Recipe

Run-on buffer (5X)

25 mM Tris-Cl (pH 8.0)

12.5 mM MgCl2

750 mM KCl

1.25 mM ATP

1.25 mM GTP

1.25 mM CTP

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  1. doi:10.1101/pdb.rec12022 Cold Spring Harb Protoc 2009: pdb.rec12022-
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