Table

Table 1. Methods of fixation

Fixation condition Permeabilization condition Types of antigens References
Formaldehyde Methanol/acetone Most proteins Adams and Pringle (1984); Kilmartin and Adams (1984)
Formaldehyde NP-40 Nuclear pore proteins Davis and Fink (1990)
Paraformaldehyde Methanol/acetone Tubulin Kilmartin and Adams (1984)
None Methanol/acetone Spindle proteins Rout and Kilmartin (1990); Osborne et al. (1994)
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  1. doi:10.1101/pdb.tab195317 Cold Spring Harb Protoc 2009: pdb.tab195317-
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