Table 1. Methods of fixation
| Fixation condition | Permeabilization condition | Types of antigens | References |
|---|---|---|---|
| Formaldehyde | Methanol/acetone | Most proteins | Adams and Pringle (1984); Kilmartin and Adams (1984) |
| Formaldehyde | NP-40 | Nuclear pore proteins | Davis and Fink (1990) |
| Paraformaldehyde | Methanol/acetone | Tubulin | Kilmartin and Adams (1984) |
| None | Methanol/acetone | Spindle proteins | Rout and Kilmartin (1990); Osborne et al. (1994) |
