Arrested C. elegans embryos injected with lmn-1 dsRNA

The expression of the lmn-1 gene (Liu J, Rolef Ben-Shahar T, Riemer D, Treinin M, Spann P, Weber K, Fire A, Gruenbaum Y. 2000. The Caenorhabditis elegans lamin gene is essential and is required for nuclear organization, mitotic progression, chromosome segregation and spatial organization of nuclear pore complexes. Mol Cell Biol 11: 3937-3947) was down-regulated in C. elegans hermaphrodites expressing histone H2B-GFP (AZ212 line; kindly provided by Dr. J. Austin [Praitis V, Casey E, Collar D, Austin J. 2001. Creation of low-copy integrated transgenic lines in Caenorhabditis elegans. Genetics 157: 1217-1226]). The F₁ progeny of hermaphrodites injected with double-stranded RNA of lmn-1 were mounted on an agar pad with egg salts solution, sealed with vacuum grease, and viewed with a Zeiss Axioplan 2 microscope equipped with an epifluorescence illuminator. An AxioCam charge-coupled device camera and the AxioVision Image Analysis package were used to collect the time-lapse data every 30 sec. The movie shows that most nuclei have disorganized chromatin. One nucleus shows the typical anaphase bridges during mitosis.Supplemental movie from Live Cell Imaging, 2nd ed., Cold Spring Harbor Laboratory Press.