FRAP experiment on a C. elegans nucleus expressing GFP::Ce-lamin wild-type protein

The movie was filmed with an FV-1000 confocal microscope (Olympus) equipped with an IX81 inverted microscope and a 60X/1.4 oil-immersion objective. Worms expressing GFP::Ce-lamin wild type were photobleached in defined regions of specific nuclei with 100% power of a 488-nm laser and imaged with a 488-nm laser line for excitation and a 505-525-nm filter for emission. Five frames every 30 sec were taken before the photobleach, 10 frames were taken every 30 sec after the photobleach, and 30 frames were taken every 2.5 min (Wiesel N, Mattout A, Melcer S, Melamed-Book N, Herrmann H, Medalia O, Aebi U, Gruenbaum Y. 2008. Laminopathic mutations interfere with the assembly, localization, and dynamics of nuclear lamins. Proc Natl Acad Sci 105: 180-185). Supplemental movie from Live Cell Imaging, 2nd ed., Cold Spring Harbor Laboratory Press.