Epifluorescence (red) and TIRFM (green) of the same image plane of vesicular stomatitis virus glycoprotein (VSVG)-YFP-tagged vesicles undergoing exocytosis

Note that red vesicles exit the Golgi, traffic to the periphery, morphologically dock (turning greener), and give a bright green flash as they fuse. Acquired sequentially at 1 Hz. (See Toomre D, Steyer JA, Keller P, Almers W, Simons K. 2000. Fusion of constitutive membrane traffic with the cell surface observed by evanescent wave microscopy. J Cell Biol 149: 33-40.) Supplemental movie from Live Cell Imaging, 2nd ed., Cold Spring Harbor Laboratory Press.