Whole-Mount Immunohistochemical Staining of Tammar Wallaby (Macropus eugenii) Peri-gastrulation Embryos
- ↵1Corresponding author (m.renfree{at}unimelb.edu.au).
INTRODUCTION
This protocol describes a method for whole-mount immunohistochemical staining of tammar wallaby (Macropus eugenii) peri-gastrulation stage embryos using the avidin-biotin complex system. Tammar embryos are ideal for whole-mount immunostaining because of their morphology: They develop in a planar manner on the surface of the embryonic vesicle and are not covered by any extraembryonic membranes until amnion formation (which occurs during mid-somitogenesis). Tammar embryos are surrounded by a transparent shell coat, which must be opened prior to staining to ensure penetration of solutions and reagents.
