Recipe

D-R buffer C

0.2 mM EDTA

25% glycerol (v/v)

20 mM HEPES-KOH (pH 7.9)

0.42 M NaCl

1.5 mM MgCl2

Immediately before use, add:

0.5 mM DTT (dithiothreitol)

0.5 mM PMSF (phenylmethylsulfonyl fluoride)

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  1. doi:10.1101/pdb.rec11241 Cold Spring Harb Protoc 2009: pdb.rec11241-
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