Recipe

D-R buffer D

0.2 mM EDTA

20% glycerol (v/v)

20 mM HEPES-KOH (pH 7.9)

0.1 M KCl

Immediately before use, add:

1 mM DTT (dithiothreitol)

1 mM PMSF (phenylmethylsulfonyl fluoride)

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  1. doi:10.1101/pdb.rec12025 Cold Spring Harb Protoc 2009: pdb.rec12025-
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