Phosphate-buffered saline for immunohistochemistry (10X)
| Reagent | Quantity (for 1 L) | Final concentration |
|---|---|---|
| Na2HPO4 (anhydrous) | 10.9 g | 77 mM |
| NaH2PO4 (anhydrous) | 3.2 g | 23 mM |
| NaCl | 90 g | 1.5 M |
| H2O | 1000 mL | |
| Mix to dissolve and adjust pH to 7.2. Store stock solution at room temperature for up to 2 mo. Before use, dilute 1:10 in H2O and readjust pH to 7.2, if necessary. Working solution is stable for up to 2 wk at room temperature. | ||
