Recipe

DABCO/PI mountant

Reagent Quantity (for 100 mL) Final concentration
DABCO (1,4-diazabicyclo-[2,2,2]-octane) 2.5 g 2.5%
Glycerol 90 mL 90%
Phosphate-buffered saline for immunohistochemistry (10X), diluted to 1X 10 mL
Propidium iodide (PI) 20 μg 0.00002%
Dissolve DABCO powder in glycerol at 37°C for ~8 h. Once dissolved, add PBS (pH 8.6). Add PI. Aliquot, wrap in foil and store at 4°C for several months.
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  1. doi:10.1101/pdb.rec12034 Cold Spring Harb Protoc 2009: pdb.rec12034-
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