Recipe

Pronase for antigen retrieval

Reagent Quantity (for 50 mL) Final concentration
Pronase 25 mg 0.05%
Phosphate-buffered saline for immunohistochemistry (10X), diluted to 1X 50 mL
Dissolve, aliquot, and store at −20°C for up to 2 yr. Warm to 37°C before use.
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  1. doi:10.1101/pdb.rec12037 Cold Spring Harb Protoc 2009: pdb.rec12037-
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