Recipe

DAB for whole-mount immunohistochemistry

Reagent Quantity (for 100 mL) Final concentration
DAB (3,3′-diaminobenzidine tetrahydrochloride) 60 mg 0.06%
TBS for immunohistochemistry (10X), diluted to 1X 100 mL
Dissolve DAB powder in 1X TBS. Dispense into 10-mL aliquots and store at −20ºC.
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  1. doi:10.1101/pdb.rec12045 Cold Spring Harb Protoc 2009: pdb.rec12045-
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