Recipe

Hybridization buffer for Ciona (HB)

Reagent Quantity (for 50 mL) Final concentration
Formamide (100%) 25.0 mL 50% (v/v)
Denhardt’s reagent (50X) 1.0 mL 1X
SSC (20X) 15.0 mL 6X
Yeast tRNA (10 mg/mL) 0.5 mL 100 μg/mL
Heparin (1%, w/v) 250 μL 0.005% (w/v)
Tween 20 (100%) 25 μL 0.05% (v/v)
H2O to 50 mL
Hybridization buffer can be stored at -20°C for a long period of time (i.e., at least 1 yr).
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  1. doi:10.1101/pdb.rec12054 Cold Spring Harb Protoc 2009: pdb.rec12054-
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