Recipe

Wash buffer 2 for Ciona (WB2)

Reagent Quantity (for 50 mL) Final concentration
Formamide (100%) 25.0 mL 50% (v/v)
SSC (20X) 12.5 mL 5X
Tween 20 (100%) 50 μL 0.1% (v/v)
H2O 12.45 mL
This can be stored at room temperature, but needs to be prewarmed to 55°C before use.
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  1. doi:10.1101/pdb.rec12062 Cold Spring Harb Protoc 2009: pdb.rec12062-
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