Recipe

NBT-BCIP-PVA staining solution

Reagent Quantity (for ~1 mL) Final concentration
NBT (4-nitro blue tetrazolium chloride) (100 μg/mL) 4.5 μL 0.45 μg/mL
BCIP (5-bromo-4-chloro-3-indolyl-phosphate) (50 mg/mL) 3.5 μL 0.17 mg/mL
PVA (10%) in TMN 1 mL
Combine the ingredients and use the solution immediately; do not store.
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  1. doi:10.1101/pdb.rec12065 Cold Spring Harb Protoc 2009: pdb.rec12065-
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