Recipe

TNB (Tris-NaCl-blocking buffer)

Reagent Quantity (for 50 mL) Final concentration
Tris-Cl (1 M, pH 7.5) 5.0 mL 0.1 M
NaCl (5 M) 1.5 mL 150 mM
Blocking reagent for Ciona (10%) 2.5 mL 0.5% (w/v)
H2O to 50 mL
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  1. doi:10.1101/pdb.rec12067 Cold Spring Harb Protoc 2009: pdb.rec12067-
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