Chemical and UV Mutagenesis of Spores and Protonemal Tissue from the Moss Physcomitrella patens
- David J. Cove,
- Pierre-François Perroud,
- Audra J. Charron,
- Stuart F. McDaniel,
- Abha Khandelwal and
- Ralph S. Quatrano1
- ↵1Corresponding author (rsq{at}wustl.edu)
INTRODUCTION
This protocol describes how to mutagenize spores and protonemal tissue from moss (Physcomitrella patens) using chemicals or ultraviolet (UV) light. Spores are mutagenized using the alkylating agents N-methyl-N′-nitro-N-nitrosoguanidine (NTG) and ethyl methanesulfonate (EMS), and protonemal tissue is mutagenized with NTG and UV light. Compared to alkylating agents, UV is less effective as a mutagen, but it may be advantageous because it is less hazardous and may not lead to clustered lesions.
