Protocol

Transformation of Moss Physcomitrella patens Gametophytes Using a Biolistic Projectile Delivery System

  1. Ralph S. Quatrano1
  1. Department of Biology, Washington University, St. Louis, MO 63130, USA
  1. ↵1Corresponding author (rsq{at}wustl.edu)
This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.

INTRODUCTION

This method makes use of biolistic bombardment to propel gold particles coated with DNA into protonemal tissue of the moss Physcomitrella patens. It is especially suitable for transient gene expression studies, but it can be used to obtain stable transformants.

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  1. doi:10.1101/pdb.prot5145 Cold Spring Harb Protoc 2009: pdb.prot5145-
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