Protocol

Whole-Mount Immunohistochemistry of Short-Tailed Fruit Bat (Carollia perspicillata)

  1. Richard R. Behringer3,4
  1. 1 Department of Obstetrics and Gynecology, State University of New York Downstate Medical Center, Brooklyn, NY 11203, USA
  2. 2 Department of Biological Sciences, Idaho State University, Pocatello, ID 83209, USA
  3. 3 Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA
  1. ↵4Corresponding author (rrb{at}mdanderson.org)
This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.

INTRODUCTION

This protocol describes whole-mount immunohistochemistry of short-tailed fruit bat (Carollia perspicillata) embryos. This method is used for direct visualization of the expression pattern of a protein of interest within embryos fixed at a wide range of developmental stages. It has been used with good results on embryos at stages from CS 11 through CS 15.

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  1. doi:10.1101/pdb.prot5167 Cold Spring Harb Protoc 2009: pdb.prot5167-
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