Recipe

Carbonate buffer (5X)

Reagent Quantity (for 10 mL) Final concentration
Sodium bicarbonate (1 M) 2.0 mL 200 mM
Sodium carbonate (1 M) 3.33 mL 300 mM
Adjust the final volume to 10 mL with H2O.

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  1. doi:10.1101/pdb.rec11675 Cold Spring Harb Protoc 2009: pdb.rec11675-
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