Recipe

DEPC-treated H2O, sterile

DEPC (diethyl pyrocarbonate; e.g., Sigma-Aldrich)

Add 1 mL of DEPC to 1 L of H2O and shake vigorously to mix. Autoclave for 20 min on liquid cycle to sterilize and deactivate DEPC.

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  1. doi:10.1101/pdb.rec11676 Cold Spring Harb Protoc 2009: pdb.rec11676-
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