Recipe

Hybridization buffer for bat embryos

Reagent Quantity (for 100 mL) Final concentration
SSC (20X, pH 5.0) 10 mL 2X
SDS (20%, pH 5.4) 5 mL 1%
Heparin (50 mg/mL) 0.1 mL 50 μg/mL
Yeast (torula) RNA (Sigma-Aldrich) (20 mg/mL) 0.25 mL 50 μg/mL
Formamide 50 mL 50%
DEPC-treated H2O, sterile to 100 mL
Store at −20°C.

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  1. doi:10.1101/pdb.rec11677 Cold Spring Harb Protoc 2009: pdb.rec11677-
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