Recipe

NTMT for bat embryos

Reagent Quantity (for 100 mL) Final concentration
NaCl (5 M) 2 mL 100 mM
Tris-Cl (1 M, pH 9.5) 10 mL 100 mM
MgCl2 (1 M) 5 mL 50 mM
Tween 20 0.1 mL 0.1%
Levamisole 48 mg 2 mM
DEPC-treated H2O, sterile to 100 mL
Prepare fresh before each use.

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  1. doi:10.1101/pdb.rec11679 Cold Spring Harb Protoc 2009: pdb.rec11679-
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