Recipe

Immune complex wash buffers

Reagent High salt Low salt
EDTA 2 mM 2 mM
NaCl 500 mM 150 mM
SDS (sodium dodecyl sulfate) 0.1% 0.1%
Tris-Cl (pH 8.1) 20 mM 20 mM
Triton X-100 1% 1%

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  1. doi:10.1101/pdb.rec11682 Cold Spring Harb Protoc 2009: pdb.rec11682-
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