OCT/sucrose mixture

OCT (Tissue-Tek)

Phosphate-buffered saline (PBS) (1X; pH 7.4), prepared without CaCl₂ or MgCl₂

Sucrose

• 1. Prepare 1X PBS containing 30% sucrose.

• 2. Combine 1:1 (v/v) OCT and 1X PBS containing 30% sucrose.