Phosphate-buffered saline (PBS), RNase-free

Phosphate-buffered saline (PBS)

Before autoclaving the PBS, add 1 mL of DEPC (diethyl pyrocarbonate) to 1 L of PBS and shake vigorously. Then, autoclave for 20 min on liquid cycle to sterilize the solution and inactivate the DEPC.

RNase-free PBS can also be purchased as a 1X solution or a 10X stock from a variety of sources.