Recipe

Medium for 3T3 cells

Donor bovine serum, heat-inactivated (10%; GIBCO)

Dulbecco’s modified Eagle medium, high-glucose (1X; GIBCO 11965)

Glutamine (2 mM; GIBCO)

Penicillin/streptomycin (100 U/mL and 100 μg/mL, respectively; GIBCO)

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  1. doi:10.1101/pdb.rec11710 Cold Spring Harb Protoc 2009: pdb.rec11710-
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